beta estradiol Search Results


β estradiol  (Tocris)
93
Tocris β estradiol
Artificial TF induction of GFP in yeast. We previously described a system to induce an artificial ZF-EV with <t>β-estradiol</t> . Activation of GFP can be induced with β-estradiol if a target complementary to the zinc finger used is placed in the promoter upstream of the GFP coding sequence. To provide a sense of relative specificity and affinity, the helices described in , and the GAG-binding fingers described in , were challenged with a set of similar targets, listed across the bottom of the chart. Each experiment included a positive control, the Zif268 activity when paired with its consensus target. This allows for the GFP output to be normalized in each experiment to the positive control output. Here, the normalized GFP output for each zinc finger and binding site pair is shown as a heat plot. For reference, a key is provided that shows the normalized GFP output when Zif268 is paired with binding sites of known affinity relative to the consensus . Asterisk: helices underlined and in italics were tested as suggested in the literature to bind the target noted on the left .
β Estradiol, supplied by Tocris, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/beta+estradiol/pmc03919609-162-7-8?v=Tocris
Average 93 stars, based on 1 article reviews
β estradiol - by Bioz Stars, 2026-07
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β estradiol  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology β estradiol
Artificial TF induction of GFP in yeast. We previously described a system to induce an artificial ZF-EV with <t>β-estradiol</t> . Activation of GFP can be induced with β-estradiol if a target complementary to the zinc finger used is placed in the promoter upstream of the GFP coding sequence. To provide a sense of relative specificity and affinity, the helices described in , and the GAG-binding fingers described in , were challenged with a set of similar targets, listed across the bottom of the chart. Each experiment included a positive control, the Zif268 activity when paired with its consensus target. This allows for the GFP output to be normalized in each experiment to the positive control output. Here, the normalized GFP output for each zinc finger and binding site pair is shown as a heat plot. For reference, a key is provided that shows the normalized GFP output when Zif268 is paired with binding sites of known affinity relative to the consensus . Asterisk: helices underlined and in italics were tested as suggested in the literature to bind the target noted on the left .
β Estradiol, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/beta+estradiol/pmc12902740-66-14-15?v=Santa+Cruz+Biotechnology
Average 93 stars, based on 1 article reviews
β estradiol - by Bioz Stars, 2026-07
93/100 stars
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β estradiol  (Thermo Fisher)
94
Thermo Fisher β estradiol
Artificial TF induction of GFP in yeast. We previously described a system to induce an artificial ZF-EV with <t>β-estradiol</t> . Activation of GFP can be induced with β-estradiol if a target complementary to the zinc finger used is placed in the promoter upstream of the GFP coding sequence. To provide a sense of relative specificity and affinity, the helices described in , and the GAG-binding fingers described in , were challenged with a set of similar targets, listed across the bottom of the chart. Each experiment included a positive control, the Zif268 activity when paired with its consensus target. This allows for the GFP output to be normalized in each experiment to the positive control output. Here, the normalized GFP output for each zinc finger and binding site pair is shown as a heat plot. For reference, a key is provided that shows the normalized GFP output when Zif268 is paired with binding sites of known affinity relative to the consensus . Asterisk: helices underlined and in italics were tested as suggested in the literature to bind the target noted on the left .
β Estradiol, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/beta+estradiol/pmc09857211-132-15-19?v=Thermo+Fisher
Average 94 stars, based on 1 article reviews
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instructions 17 β estradiol e2  (Tocris)
94
Tocris instructions 17 β estradiol e2
Artificial TF induction of GFP in yeast. We previously described a system to induce an artificial ZF-EV with <t>β-estradiol</t> . Activation of GFP can be induced with β-estradiol if a target complementary to the zinc finger used is placed in the promoter upstream of the GFP coding sequence. To provide a sense of relative specificity and affinity, the helices described in , and the GAG-binding fingers described in , were challenged with a set of similar targets, listed across the bottom of the chart. Each experiment included a positive control, the Zif268 activity when paired with its consensus target. This allows for the GFP output to be normalized in each experiment to the positive control output. Here, the normalized GFP output for each zinc finger and binding site pair is shown as a heat plot. For reference, a key is provided that shows the normalized GFP output when Zif268 is paired with binding sites of known affinity relative to the consensus . Asterisk: helices underlined and in italics were tested as suggested in the literature to bind the target noted on the left .
Instructions 17 β Estradiol E2, supplied by Tocris, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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instructions 17 β estradiol e2 - by Bioz Stars, 2026-07
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h estradiol 17 β d glucuronide  (Revvity)
91
Revvity h estradiol 17 β d glucuronide
Artificial TF induction of GFP in yeast. We previously described a system to induce an artificial ZF-EV with <t>β-estradiol</t> . Activation of GFP can be induced with β-estradiol if a target complementary to the zinc finger used is placed in the promoter upstream of the GFP coding sequence. To provide a sense of relative specificity and affinity, the helices described in , and the GAG-binding fingers described in , were challenged with a set of similar targets, listed across the bottom of the chart. Each experiment included a positive control, the Zif268 activity when paired with its consensus target. This allows for the GFP output to be normalized in each experiment to the positive control output. Here, the normalized GFP output for each zinc finger and binding site pair is shown as a heat plot. For reference, a key is provided that shows the normalized GFP output when Zif268 is paired with binding sites of known affinity relative to the consensus . Asterisk: helices underlined and in italics were tested as suggested in the literature to bind the target noted on the left .
H Estradiol 17 β D Glucuronide, supplied by Revvity, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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e2 ab biorad cat 7010 2650 lot 0113 h3 labeled e2 perkin elmer net517250uc  (Bio-Rad)
91
Bio-Rad e2 ab biorad cat 7010 2650 lot 0113 h3 labeled e2 perkin elmer net517250uc
Artificial TF induction of GFP in yeast. We previously described a system to induce an artificial ZF-EV with <t>β-estradiol</t> . Activation of GFP can be induced with β-estradiol if a target complementary to the zinc finger used is placed in the promoter upstream of the GFP coding sequence. To provide a sense of relative specificity and affinity, the helices described in , and the GAG-binding fingers described in , were challenged with a set of similar targets, listed across the bottom of the chart. Each experiment included a positive control, the Zif268 activity when paired with its consensus target. This allows for the GFP output to be normalized in each experiment to the positive control output. Here, the normalized GFP output for each zinc finger and binding site pair is shown as a heat plot. For reference, a key is provided that shows the normalized GFP output when Zif268 is paired with binding sites of known affinity relative to the consensus . Asterisk: helices underlined and in italics were tested as suggested in the literature to bind the target noted on the left .
E2 Ab Biorad Cat 7010 2650 Lot 0113 H3 Labeled E2 Perkin Elmer Net517250uc, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/beta+estradiol/pm37524232-93-17-19?v=Bio-Rad
Average 91 stars, based on 1 article reviews
e2 ab biorad cat 7010 2650 lot 0113 h3 labeled e2 perkin elmer net517250uc - by Bioz Stars, 2026-07
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estradiol e2  (Toronto Research Chemicals)
93
Toronto Research Chemicals estradiol e2
Multiple reaction monitoring parameters for steroids using LC/MS/MS
Estradiol E2, supplied by Toronto Research Chemicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/beta+estradiol/pmc07112139-126-20-32?v=Toronto+Research+Chemicals
Average 93 stars, based on 1 article reviews
estradiol e2 - by Bioz Stars, 2026-07
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plasma 17β estradiol concentrations  (ALPCO)
88
ALPCO plasma 17β estradiol concentrations
Multiple reaction monitoring parameters for steroids using LC/MS/MS
Plasma 17β Estradiol Concentrations, supplied by ALPCO, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 88 stars, based on 1 article reviews
plasma 17β estradiol concentrations - by Bioz Stars, 2026-07
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anti 17β estradiol antibody  (Santa Cruz Biotechnology)
92
Santa Cruz Biotechnology anti 17β estradiol antibody
Multiple reaction monitoring parameters for steroids using LC/MS/MS
Anti 17β Estradiol Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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estradiol 17 β  (Toronto Research Chemicals)
91
Toronto Research Chemicals estradiol 17 β
Multiple reaction monitoring parameters for steroids using LC/MS/MS
Estradiol 17 β, supplied by Toronto Research Chemicals, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 91 stars, based on 1 article reviews
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β estradiol 3 sulfate sodium salt  (Santa Cruz Biotechnology)
92
Santa Cruz Biotechnology β estradiol 3 sulfate sodium salt
Protein expression and enzymatic activity of SULT1E1 in the liver of MRP2-deficient EHBR. (A) The protein expression of SULT1E1 in the liver of 5-week-old control SD rats and EHBR was evaluated using Western blotting. The quantified relative protein expression was normalized to the expression of β -actin as the loading control. (B) The enzymatic activity of SULT1E1 was evaluated using liver cytosol and β -estradiol as a typical substrate. The formed concentration of a sulfated metabolite, β -estradiol <t>3-sulfate,</t> was quantified by LC-MS/MS. The data for β -estradiol 3-sulfate formation rates were fitted to Michaelis–Menten equation. (C, D) Sulfation of fulvestrant (C) and resveratrol (D) was evaluated using liver cytosol. The formation of sulfated metabolites was assessed based on LC-MS/MS peak areas. Data are expressed as mean ± S.D. ( n = 5). ∗ P < .05, ∗∗ P < .01, ∗∗∗ P < .001 vs SD rats (Student’s t test). EHBR, Eisai hyperbilirubinemic rats; MRP, multidrug resistance–associated protein; SULT, sulfotransferase.
β Estradiol 3 Sulfate Sodium Salt, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/beta+estradiol/pmc12799537-103-13-18?v=Santa+Cruz+Biotechnology
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gapdh  (Boster Bio)
94
Boster Bio gapdh
Protein expression and enzymatic activity of SULT1E1 in the liver of MRP2-deficient EHBR. (A) The protein expression of SULT1E1 in the liver of 5-week-old control SD rats and EHBR was evaluated using Western blotting. The quantified relative protein expression was normalized to the expression of β -actin as the loading control. (B) The enzymatic activity of SULT1E1 was evaluated using liver cytosol and β -estradiol as a typical substrate. The formed concentration of a sulfated metabolite, β -estradiol <t>3-sulfate,</t> was quantified by LC-MS/MS. The data for β -estradiol 3-sulfate formation rates were fitted to Michaelis–Menten equation. (C, D) Sulfation of fulvestrant (C) and resveratrol (D) was evaluated using liver cytosol. The formation of sulfated metabolites was assessed based on LC-MS/MS peak areas. Data are expressed as mean ± S.D. ( n = 5). ∗ P < .05, ∗∗ P < .01, ∗∗∗ P < .001 vs SD rats (Student’s t test). EHBR, Eisai hyperbilirubinemic rats; MRP, multidrug resistance–associated protein; SULT, sulfotransferase.
Gapdh, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/beta+estradiol/pmc12979763-85-31-32?v=Boster+Bio
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Image Search Results


Artificial TF induction of GFP in yeast. We previously described a system to induce an artificial ZF-EV with β-estradiol . Activation of GFP can be induced with β-estradiol if a target complementary to the zinc finger used is placed in the promoter upstream of the GFP coding sequence. To provide a sense of relative specificity and affinity, the helices described in , and the GAG-binding fingers described in , were challenged with a set of similar targets, listed across the bottom of the chart. Each experiment included a positive control, the Zif268 activity when paired with its consensus target. This allows for the GFP output to be normalized in each experiment to the positive control output. Here, the normalized GFP output for each zinc finger and binding site pair is shown as a heat plot. For reference, a key is provided that shows the normalized GFP output when Zif268 is paired with binding sites of known affinity relative to the consensus . Asterisk: helices underlined and in italics were tested as suggested in the literature to bind the target noted on the left .

Journal: Nucleic Acids Research

Article Title: Deep sequencing of large library selections allows computational discovery of diverse sets of zinc fingers that bind common targets

doi: 10.1093/nar/gkt1034

Figure Lengend Snippet: Artificial TF induction of GFP in yeast. We previously described a system to induce an artificial ZF-EV with β-estradiol . Activation of GFP can be induced with β-estradiol if a target complementary to the zinc finger used is placed in the promoter upstream of the GFP coding sequence. To provide a sense of relative specificity and affinity, the helices described in , and the GAG-binding fingers described in , were challenged with a set of similar targets, listed across the bottom of the chart. Each experiment included a positive control, the Zif268 activity when paired with its consensus target. This allows for the GFP output to be normalized in each experiment to the positive control output. Here, the normalized GFP output for each zinc finger and binding site pair is shown as a heat plot. For reference, a key is provided that shows the normalized GFP output when Zif268 is paired with binding sites of known affinity relative to the consensus . Asterisk: helices underlined and in italics were tested as suggested in the literature to bind the target noted on the left .

Article Snippet: Induction of ZF-EV activators by 100 nM β-estradiol (Tocris Biosciences, Ellisville, MO, USA) was performed in cells during log-phase growth (culture absorbance = 50–100 Klett units).

Techniques: Activation Assay, Sequencing, Binding Assay, Positive Control, Activity Assay

Multiple reaction monitoring parameters for steroids using LC/MS/MS

Journal: Journal of Lipid Research

Article Title: An LC/MS/MS method for analyzing the steroid metabolome with high accuracy and from small serum samples [S]

doi: 10.1194/jlr.D119000591

Figure Lengend Snippet: Multiple reaction monitoring parameters for steroids using LC/MS/MS

Article Snippet: Pregnenolone (Preg), pregnenolone-17,21,21,21-d4 (Preg-d4), 17-hydroxypregnenolone (17OHPreg), 17-hydroxypregnenolone-21,21,21-d3 (17OHPreg-d3), 17-hydroxyprogesterone (17OHP), 17-hydroxyprogesterone-2,2,4,6,6,21,21,21-d8 (17OHP-d8), corticosterone (CORT), corticosterone-2,2,4,6,6,17,21,21-d8 (CORT-d8), cortisol (COR), 11-deoxycortisol (DOC), estradiol (E2), estriol (E3), estriol-2,4,17-d3 (E3-d3), and progesterone-2,2,4,6,6,17,21,21,21-d9 (P-d9) were purchased from Toronto Research Chemicals (Toronto, Canada).

Techniques: Targeted Proteomics

Intra- and interassay precisions of steroid metabolome analysis

Journal: Journal of Lipid Research

Article Title: An LC/MS/MS method for analyzing the steroid metabolome with high accuracy and from small serum samples [S]

doi: 10.1194/jlr.D119000591

Figure Lengend Snippet: Intra- and interassay precisions of steroid metabolome analysis

Article Snippet: Pregnenolone (Preg), pregnenolone-17,21,21,21-d4 (Preg-d4), 17-hydroxypregnenolone (17OHPreg), 17-hydroxypregnenolone-21,21,21-d3 (17OHPreg-d3), 17-hydroxyprogesterone (17OHP), 17-hydroxyprogesterone-2,2,4,6,6,21,21,21-d8 (17OHP-d8), corticosterone (CORT), corticosterone-2,2,4,6,6,17,21,21-d8 (CORT-d8), cortisol (COR), 11-deoxycortisol (DOC), estradiol (E2), estriol (E3), estriol-2,4,17-d3 (E3-d3), and progesterone-2,2,4,6,6,17,21,21,21-d9 (P-d9) were purchased from Toronto Research Chemicals (Toronto, Canada).

Techniques:

Certified and measured values of steroids in the MassCheck® Steroid Panel Serum Control

Journal: Journal of Lipid Research

Article Title: An LC/MS/MS method for analyzing the steroid metabolome with high accuracy and from small serum samples [S]

doi: 10.1194/jlr.D119000591

Figure Lengend Snippet: Certified and measured values of steroids in the MassCheck® Steroid Panel Serum Control

Article Snippet: Pregnenolone (Preg), pregnenolone-17,21,21,21-d4 (Preg-d4), 17-hydroxypregnenolone (17OHPreg), 17-hydroxypregnenolone-21,21,21-d3 (17OHPreg-d3), 17-hydroxyprogesterone (17OHP), 17-hydroxyprogesterone-2,2,4,6,6,21,21,21-d8 (17OHP-d8), corticosterone (CORT), corticosterone-2,2,4,6,6,17,21,21-d8 (CORT-d8), cortisol (COR), 11-deoxycortisol (DOC), estradiol (E2), estriol (E3), estriol-2,4,17-d3 (E3-d3), and progesterone-2,2,4,6,6,17,21,21,21-d9 (P-d9) were purchased from Toronto Research Chemicals (Toronto, Canada).

Techniques:

Baseline characteristics and steroid concentrations of pregnant women

Journal: Journal of Lipid Research

Article Title: An LC/MS/MS method for analyzing the steroid metabolome with high accuracy and from small serum samples [S]

doi: 10.1194/jlr.D119000591

Figure Lengend Snippet: Baseline characteristics and steroid concentrations of pregnant women

Article Snippet: Pregnenolone (Preg), pregnenolone-17,21,21,21-d4 (Preg-d4), 17-hydroxypregnenolone (17OHPreg), 17-hydroxypregnenolone-21,21,21-d3 (17OHPreg-d3), 17-hydroxyprogesterone (17OHP), 17-hydroxyprogesterone-2,2,4,6,6,21,21,21-d8 (17OHP-d8), corticosterone (CORT), corticosterone-2,2,4,6,6,17,21,21-d8 (CORT-d8), cortisol (COR), 11-deoxycortisol (DOC), estradiol (E2), estriol (E3), estriol-2,4,17-d3 (E3-d3), and progesterone-2,2,4,6,6,17,21,21,21-d9 (P-d9) were purchased from Toronto Research Chemicals (Toronto, Canada).

Techniques:

Protein expression and enzymatic activity of SULT1E1 in the liver of MRP2-deficient EHBR. (A) The protein expression of SULT1E1 in the liver of 5-week-old control SD rats and EHBR was evaluated using Western blotting. The quantified relative protein expression was normalized to the expression of β -actin as the loading control. (B) The enzymatic activity of SULT1E1 was evaluated using liver cytosol and β -estradiol as a typical substrate. The formed concentration of a sulfated metabolite, β -estradiol 3-sulfate, was quantified by LC-MS/MS. The data for β -estradiol 3-sulfate formation rates were fitted to Michaelis–Menten equation. (C, D) Sulfation of fulvestrant (C) and resveratrol (D) was evaluated using liver cytosol. The formation of sulfated metabolites was assessed based on LC-MS/MS peak areas. Data are expressed as mean ± S.D. ( n = 5). ∗ P < .05, ∗∗ P < .01, ∗∗∗ P < .001 vs SD rats (Student’s t test). EHBR, Eisai hyperbilirubinemic rats; MRP, multidrug resistance–associated protein; SULT, sulfotransferase.

Journal: Drug Metabolism and Disposition

Article Title: Downregulation of hepatic sulfotransferase 1E1 expression associated with decreased expression of multidrug resistance–associated protein 2

doi: 10.1016/j.dmd.2025.100190

Figure Lengend Snippet: Protein expression and enzymatic activity of SULT1E1 in the liver of MRP2-deficient EHBR. (A) The protein expression of SULT1E1 in the liver of 5-week-old control SD rats and EHBR was evaluated using Western blotting. The quantified relative protein expression was normalized to the expression of β -actin as the loading control. (B) The enzymatic activity of SULT1E1 was evaluated using liver cytosol and β -estradiol as a typical substrate. The formed concentration of a sulfated metabolite, β -estradiol 3-sulfate, was quantified by LC-MS/MS. The data for β -estradiol 3-sulfate formation rates were fitted to Michaelis–Menten equation. (C, D) Sulfation of fulvestrant (C) and resveratrol (D) was evaluated using liver cytosol. The formation of sulfated metabolites was assessed based on LC-MS/MS peak areas. Data are expressed as mean ± S.D. ( n = 5). ∗ P < .05, ∗∗ P < .01, ∗∗∗ P < .001 vs SD rats (Student’s t test). EHBR, Eisai hyperbilirubinemic rats; MRP, multidrug resistance–associated protein; SULT, sulfotransferase.

Article Snippet: For quantity, an absolute calibration method was used and a standard product of β -estradiol 3-sulfate sodium salt (Santa Cruz Biotechnology, Inc) was used for the standard.

Techniques: Expressing, Activity Assay, Control, Western Blot, Concentration Assay, Liquid Chromatography with Mass Spectroscopy